Time & Place: January 7-18, Monday-Friday, 4-5:30p, Room 46-3015
Sign-up: Please use the sign-up form to register for the class.
Course description: Why do people act the way that they do? How sensory input alters the behavioral output of living organisms is a fascinating question in neuroscience. While this is difficult to study in a gap-free manner at the cellular level in mammals, gap-free neural circuits have been identified and their signal transformation properties characterized in simpler organisms. On each day of this class we will discuss a single neural circuit that has been worked out at the cellular level, including how each neuron in the circuit transforms the incoming physiological signal using specific molecules. Circuits will be derived from primary experimental data. We will focus on circuits for which the neurons that sense the stimuli are known, the interneurons are known, and the motor neurons controlling muscle contraction and the resulting behavior are known. Circuits will be drawn from several invertebrate organisms, including the genetic organisms C. elegans and Drosophila, as well as the locust, crayfish and cricket. After this class students will have a precise understanding of several different neural circuits as well as the methods used to identify and analyze these circuits. By providing several examples of real neural circuits, principles for how circuits function in general may become apparent. Students, post-docs and professors welcome.
Contact: firstname.lastname@example.org & email@example.com
Introduction (Tots & Nikhil) - Notes - Circuit analysis rubric - Slides
Overview of course, philosophy and motivation, electrophysiology and imaging, circuit analysis, and other methods.
|Olsen & Wilson 2008 (*)|
Crayfish escape (Tots) - Slides - Video of crayfish escape
Fictive behavior, command neuron, local and distributed computation, rectifying electrical synapse for coincidence detection, presynaptic inhbition, proximal vs. distal dendritic inhibition.
Olson & Krasne 1981 (*)
Worm: Touch-induced locomotion (Nikhil) - Notes - Slides - Slides+videos
C. elegans-specific neural signaling, connectome-motivated hypothesis generation, ordering neurons into a circuit, convergent excitatory circuit, parallel disinhibitory circuit.
Piggott..Xu 2011 (*)
Locust flight (Tots) - Slides
Tethered flight, central pattern generators (CPGs), proprioceptive feedback, neural system for achieving stable flight, interneurons important for phase-coupling.
Reichert..Griss 1985 (*)
Reichert & Rowell 1986
Robertson & Pearson 1985
Worm: Light-induced pump stop (Nikhil) - Notes (partial) - Slides (partial)
Neural network islands, nervous system as modulatory, redundant control circuits, temporal tiling of circuit function.
Avery & Horvitz 1989 (*)
Worm: Olfaction & chemotaxis (Nikhil) - Notes - Slides - Slides+videos
Sensory processing of food odors, motor control of turning, local search for food, repetitive sign inversion in a linear chain.
Chalasani..Bargmann 2007 (*)
Kocabas..Ramanathan 2012 (*)
Fly mating decision (Tots) - Slides
Behavioral effect of pheromones, circuit tracing using PA-GFP, demonstration of functional connectivity, triggering behavior using artificially activated neurons.
Ruta..Axel 2010 (*)
Ha & Smith 2006
Worm mating (Nikhil) - Notes - Slides - Slides+videos
Neural control of male attraction to hermaphrodite pheromones, retention with mechanical stimuli, mating motor program: finding, stroking, vulva locating, penetrating, ejaculation; independent modules that form a motor sequence.
Liu & Sternberg 1995 (*)
Barr & Garcia 2006
Loer & Kenyon 1993
Cricket mating (Tots) - Notes - Slides
Biomechanics of song production in the male, corollary discharge, sound localization and pattern recognition in the female.
Poulet & Hedwig 2006 (*)
Conclusion (Tots & Nikhil)
Review of circuit analysis methods, final circuit principles, connection to mammals.
This website previously hosted at http://mit.edu/nbhatla/gapfree/